Comparison of Human Serum Dopamine- -Hydroxylase Levels by Radioimmunoassay and Enzymatic Assay
نویسندگان
چکیده
منابع مشابه
Measurement of human dopamine-beta-hydroxylase in serum by homologous radioimmunoassay.
Human serum dopamine-beta-hydroxylase [3,4-dihydroxyphenylethylamine, ascorbate: oxygen oxidoreducatse (beta-hydroxylating), EC 1.14.17.1] levels have been determined in 52 samples with a newly devised radioimmunoassay that utilizes purified human enzyme and its specific antiserum. The values obtained were in excellent agreement (correlation coefficient r equals 0.98) with those obtained by mea...
متن کاملComparison of human prostatic acid phosphatase by measurement of enzymatic activity and by radioimmunoassay.
We compared results of measurement of prostatic acid phosphatase activity in serum and various tissues by enzymatic assay and radioimmunoassay. By enzymatic assay, activity in serum is lost rapidly, even at room temperature. In contrast, there was no change in antigenic activity during 48 h by radioimmunoassay. The radioimmunoassay was more specific in 12 tissues and in serum than were several ...
متن کاملSerum dopamine-beta-hydroxylase activity.
Dopamine-yS-liydroxylase, the enzyme which converts dopamine to norepinephrine, is released into the perfusate upon stimulation of the isolated perfused adrenal gland and after stimulation of the nerves to the isolated perfused spleen. This study was undertaken to determine whether dopamine-/?hydroxylase activity could be detected circulating in blood. By using a sensitive new enzymatic assay, ...
متن کاملRadioimmunoassay of human serum thyrotrophin.
The double antibody radioimmunoassay of serum thyroid-stimulating hormone (TSH) allows measurement of circulating levels of the hormone in most normal subjects. The serum TSH level in normal subjects is 1.6 +/- 0.8muU/ml. Patients with non-toxic goitre and acromegaly have normal TSH levels. Values are always raised in hypothyroid patients (with primary thyroid disease) and are significantly low...
متن کاملComparison of a radioimmunoassay and a microbiological assay for measurement of serum vancomycin concentrations.
A newly developed radioimmunoassay was used to measure the concentration of vancomycin in 137 specimens of serum from patients being treated with this antibiotic. Of these sera, 84 were also analyzed with a microbiological assay technique for vancomycin. Duplicate determinations were done with each of the techniques. Individual values and averaged values for both methods were used for statistic...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1974
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.71.3.872